Immuno- and flow cytometric analytical methods for biotechnological research and process monitoring

verfasst von: A. Degelau, R. Freitag, F. Linz, C. Middendorf, T. Scheper, T. Bley, S. Müller, P. Stoll, K. F. Reardon
Abstract: In this article, the applications of immunoanalysis and flow cytometry for research and process monitoring in biotechnology are discussed. Brief reviews of the two analytical methods are followed by descriptions of actual applications in various areas of biotechnology. In the case of immunoanalysis, emphasis is placed on systems for on-line bioprocess monitoring, and examples are given for a thermostable pullulanase, a mouse IgG, and antithrombin III. Although flow cytometry is not currently an on-line analytical technique, its value as an off-line method is illustrated by examples of the measurement of shear stress effects, lipid content, and sterol content.
Organisationseinheit(en): Institut für Technische Chemie
Externe Organisation(en): Colorado State University
Sächsisches Institut für Angewandte Biotechnologie e.V.
Typ: Artikel
Journal: Journal of biotechnology
Band: 25
Seiten: 115-144
Anzahl der Seiten: 30
ISSN: 0168-1656
Publikationsdatum: 08.1992
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
ASJC Scopus Sachgebiete: Biotechnologie, Bioengineering, Angewandte Mikrobiologie und Biotechnologie
Elektronische Version(en): https://doi.org/10.1016/0168-1656(92)90112-M (Zugang: Unbekannt)

BibTeX

@article{16f89cec458a4e27b26ea38a6c616460,
title = "Immuno- and flow cytometric analytical methods for biotechnological research and process monitoring",
abstract = "In this article, the applications of immunoanalysis and flow cytometry for research and process monitoring in biotechnology are discussed. Brief reviews of the two analytical methods are followed by descriptions of actual applications in various areas of biotechnology. In the case of immunoanalysis, emphasis is placed on systems for on-line bioprocess monitoring, and examples are given for a thermostable pullulanase, a mouse IgG, and antithrombin III. Although flow cytometry is not currently an on-line analytical technique, its value as an off-line method is illustrated by examples of the measurement of shear stress effects, lipid content, and sterol content.",
keywords = "Bioprocess monitoring, Flow injection analysis, Immunoassay, Laser flow cytometry, On-line analysis",
author = "A. Degelau and R. Freitag and F. Linz and C. Middendorf and T. Scheper and T. Bley and S. M{\"u}ller and P. Stoll and Reardon, {K. F.}",
note = "Funding information: The authors gratefully acknowledge the support of the DFG and the BMFT (Forschungsschwerpunkt: 'Grundlagen der Bioprozesstechnik') for their cooperation.",
year = "1992",
month = aug,
doi = "10.1016/0168-1656(92)90112-M",
language = "English",
volume = "25",
pages = "115--144",
journal = "Journal of biotechnology",
issn = "0168-1656",
publisher = "Elsevier",
number = "1-2",
}