The general mitochondrial processing peptidase from potato is an integral part of cytochrome c reductase of the respiratory chain

verfasst von

Hans-Peter Braun, Michael Emmermann, Volker Kruft, Udo Schmitz

Abstract

The major mitochondrial processing activity removing presequences from nuclear encoded precursor proteins is present in the soluble fraction of fungal and mammalian mitochondria. We found that in potato, this activity resides in the inner mitochondrial membrane. Surprisingly, the proteolytic activity co-purifies with cytochrome c reductase, a protein complex of the respiratory chain. The purified complex is bifunctional, as it has the ability to transfer electrons from ubiquinol to cytochrome c and to cleave off the presequences of mitochondrial precursor proteins. In contrast to the nine subunit fungal complex, cytochrome c reductase from potato comprises 10 polypeptides. Protein sequencing of peptides from individual subunits and analysis of corresponding cDNA clones reveals that subunit III of cytochrome c reductase (51 kDa) represents the general mitochondrial processing peptidase.

Externe Organisation(en)

Max-Planck-Institut für molekulare Pflanzenphysiologie

Typ

Artikel

Journal

EMBO Journal

Band

11

Seiten

3219-3227

Anzahl der Seiten

9

ISSN

0261-4189

Publikationsdatum

1992

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Allgemeine Neurowissenschaft, Molekularbiologie, Allgemeine Biochemie, Genetik und Molekularbiologie, Allgemeine Immunologie und Mikrobiologie