Fs-laser cell perforation using gold nanoparticles of different shapes

verfasst von: Markus Schomaker, Holger Fehlauer, Willem Bintig, Anaclet Ngezahayo, Ingo Nolte, Hugo Murua Escobar, Holger Lubatschowski, Alexander Heisterkamp
Abstract: The resulting effects of the interaction between nanoparticles and laser irradiation are a current matter in research. Depending on the laser parameters as well as the particles properties several effects may occur e.g. bubble formation, melting, fragmentation or an optical breakdown at the surface of the nanoparticle. Besides the investigations of these effects, we employed them to perforate the membrane of different cell lines and investigated nanoparticle mediated laser cell perforation as an alternative optical transfection method. Therefore, the gold nanoparticles (GNP) of different shapes were applied. Furthermore, we varied the methods for attaching GNP to the membrane, i.e. co-incubation of pure gold nanoparticles and bioconjugation of the surface of GNP. The optimal incubation time and the location of the GNP at the cell membrane were evaluated by multiphoton microscopy. If these GNP loaded cells are irradiated with a fs laser beam, small areas of the membrane can be perforated. Following, extra cellular molecules such as membrane impermeable dyes or foreign DNA (GFP vectors) are able to diffuse through the perforated area into the treated cells. We studied the dependence of the laser fluence, GNP concentration, GNP size and shape for successful nanoparticle mediated laser cell perforation. Due to a weak focusing of the laser beam a gentle cell treatment with high cell viabilities and high perforation efficiencies can be achieved. A further advantage of this perforation technique is the high number of cells that can be treated simultaneously. Additionally, we show applications of this method to primary and stem cells.
Organisationseinheit(en): Institut für Zellbiologie und Biophysik
Externe Organisation(en): Laser Zentrum Hannover e.V. (LZH)
Stiftung Tierärztliche Hochschule Hannover
REBIRTH Forschungszentrum für translationale regenerative Medizin
Typ: Aufsatz in Konferenzband
Publikationsdatum: 25.02.2010
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
ASJC Scopus Sachgebiete: Elektronische, optische und magnetische Materialien, Physik der kondensierten Materie, Angewandte Informatik, Angewandte Mathematik, Elektrotechnik und Elektronik
Elektronische Version(en): https://doi.org/10.1117/12.842446 (Zugang: Unbekannt)

BibTeX

@inproceedings{c476d638eb2f4bf2b8be688e14649691,
title = "Fs-laser cell perforation using gold nanoparticles of different shapes",
abstract = "The resulting effects of the interaction between nanoparticles and laser irradiation are a current matter in research. Depending on the laser parameters as well as the particles properties several effects may occur e.g. bubble formation, melting, fragmentation or an optical breakdown at the surface of the nanoparticle. Besides the investigations of these effects, we employed them to perforate the membrane of different cell lines and investigated nanoparticle mediated laser cell perforation as an alternative optical transfection method. Therefore, the gold nanoparticles (GNP) of different shapes were applied. Furthermore, we varied the methods for attaching GNP to the membrane, i.e. co-incubation of pure gold nanoparticles and bioconjugation of the surface of GNP. The optimal incubation time and the location of the GNP at the cell membrane were evaluated by multiphoton microscopy. If these GNP loaded cells are irradiated with a fs laser beam, small areas of the membrane can be perforated. Following, extra cellular molecules such as membrane impermeable dyes or foreign DNA (GFP vectors) are able to diffuse through the perforated area into the treated cells. We studied the dependence of the laser fluence, GNP concentration, GNP size and shape for successful nanoparticle mediated laser cell perforation. Due to a weak focusing of the laser beam a gentle cell treatment with high cell viabilities and high perforation efficiencies can be achieved. A further advantage of this perforation technique is the high number of cells that can be treated simultaneously. Additionally, we show applications of this method to primary and stem cells.",
keywords = "Biophotonics, Cell manipulation, GFP, Membrane permeabilization, Nanoparticles, Nanorods, Perforation, Plasmonics, Transfection, Ultrashort laser pulses",
author = "Markus Schomaker and Holger Fehlauer and Willem Bintig and Anaclet Ngezahayo and Ingo Nolte and {Murua Escobar}, Hugo and Holger Lubatschowski and Alexander Heisterkamp",
year = "2010",
month = feb,
day = "25",
doi = "10.1117/12.842446",
language = "English",
isbn = "9780819479853",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
booktitle = "Frontiers in Ultrafast Optics",
note = "Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications X ; Conference date: 24-01-2010 Through 26-01-2010",
}