Real-time magnetic resonance imaging and quantification of lipoprotein metabolism in vivo using nanocrystals

verfasst von: Oliver T. Bruns, Harald Ittrich, Kersten Peldschus, Michael G. Kaul, Ulrich I. Tromsdorf, Joachim Lauterwasser, Marija S. Nikolic, Birgit Mollwitz, Martin Merkel, Nadja C. Bigall, Sameer Sapra, Rudolph Reimer, Heinz Hohenberg, Horst Weller, Alexander Eychmüller, Gerhard Adam, Ulrike Beisiegel, Joerg Heeren
Abstract: Semiconductor quantum dots and superparamagnetic iron oxide nanocrystals have physical properties that are well suited for biomedical imaging. Previously, we have shown that iron oxide nanocrystals embedded within the lipid core of micelles show optimized characteristics for quantitative imaging. Here, we embed quantum dots and superparamagnetic iron oxide nanocrystals in the core of lipoproteins - micelles that transport lipids and other hydrophobic substances in the blood - and show that it is possible to image and quantify the kinetics of lipoprotein metabolism in vivo using fluorescence and dynamic magnetic resonance imaging. The lipoproteins were taken up by liver cells in wild-type mice and displayed defective clearance in knock-out mice lacking a lipoprotein receptor or its ligand, indicating that the nanocrystals did not influence the specificity of the metabolic process. Using this strategy it is possible to study the clearance of lipoproteins in metabolic disorders and to improve the contrast in clinical imaging.
Externe Organisation(en): Universitätsklinikum Hamburg-Eppendorf
Universität Hamburg
Asklepios Klinik St. Georg
Technische Universität Dresden
Typ: Artikel
Journal: Nature nanotechnology
Band: 4
Seiten: 193-201
Anzahl der Seiten: 9
ISSN: 1748-3387
Publikationsdatum: 03.2009
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
ASJC Scopus Sachgebiete: Bioengineering, Atom- und Molekularphysik sowie Optik, Biomedizintechnik, Allgemeine Materialwissenschaften, Physik der kondensierten Materie, Elektrotechnik und Elektronik
Elektronische Version(en): https://doi.org/10.1038/nnano.2008.405 (Zugang: Unbekannt)

BibTeX

@article{9d37d08fc6a2457e9727d8ec740844c9,
title = "Real-time magnetic resonance imaging and quantification of lipoprotein metabolism in vivo using nanocrystals",
abstract = "Semiconductor quantum dots and superparamagnetic iron oxide nanocrystals have physical properties that are well suited for biomedical imaging. Previously, we have shown that iron oxide nanocrystals embedded within the lipid core of micelles show optimized characteristics for quantitative imaging. Here, we embed quantum dots and superparamagnetic iron oxide nanocrystals in the core of lipoproteins - micelles that transport lipids and other hydrophobic substances in the blood - and show that it is possible to image and quantify the kinetics of lipoprotein metabolism in vivo using fluorescence and dynamic magnetic resonance imaging. The lipoproteins were taken up by liver cells in wild-type mice and displayed defective clearance in knock-out mice lacking a lipoprotein receptor or its ligand, indicating that the nanocrystals did not influence the specificity of the metabolic process. Using this strategy it is possible to study the clearance of lipoproteins in metabolic disorders and to improve the contrast in clinical imaging.",
author = "Bruns, {Oliver T.} and Harald Ittrich and Kersten Peldschus and Kaul, {Michael G.} and Tromsdorf, {Ulrich I.} and Joachim Lauterwasser and Nikolic, {Marija S.} and Birgit Mollwitz and Martin Merkel and Bigall, {Nadja C.} and Sameer Sapra and Rudolph Reimer and Heinz Hohenberg and Horst Weller and Alexander Eychm{\"u}ller and Gerhard Adam and Ulrike Beisiegel and Joerg Heeren",
note = "Funding information: The authors thank A. Laatsch, R. Fischer and A. Bartelt for helpful discussions. K. Cornils, B. Holstermann, M. Warmer, S. Ehret and R. Kongi for excellent technical assistance, A. Kornowski for electron microscopy images and R. Capek for providing QD. O.T.B. is supported by a fellowship from the Studienstiftung des Deutschen Volkes. This work was supported by grants from the Deutsche Forschungsgemeinschaft to J.H., U.B. and A.E. (HE3645/2-2, BE829/10-1 and EY16/9-1).",
year = "2009",
month = mar,
doi = "10.1038/nnano.2008.405",
language = "English",
volume = "4",
pages = "193--201",
journal = "Nature nanotechnology",
issn = "1748-3387",
publisher = "Nature Publishing Group",
number = "3",
}