Optical and electron microscopy study of laser-based intracellular molecule delivery using peptide-conjugated photodispersible gold nanoparticle agglomerates

verfasst von: Judith Krawinkel, Undine Richter, Maria Leilani Torres-Mapa, Martin Westermann, Lisa Gamrad, Christoph Rehbock, Stephan Barcikowski, Alexander Heisterkamp
Abstract: Background: Cell-penetrating peptides (CPPs) can act as carriers for therapeutic molecules such as drugs and genetic constructs for medical applications. The triggered release of the molecule into the cytoplasm can be crucial to its effective delivery. Hence, we implemented and characterized laser interaction with defined gold nanoparticle agglomerates conjugated to CPPs which enables efficient endosomal rupture and intracellular release of molecules transported. Results: Gold nanoparticles generated by pulsed laser ablation in liquid were conjugated with CPPs forming agglomerates and the intracellular release of molecules was triggered via pulsed laser irradiation (λ = 532 nm, τ_pulse = 1 ns). The CPPs enhance the uptake of the agglomerates along with the cargo which can be co-incubated with the agglomerates. The interaction of incident laser light with gold nanoparticle agglomerates leads to heat deposition and field enhancement in the vicinity of the particles. This highly precise effect deagglomerates the nanoparticles and disrupts the enclosing endosomal membrane. Transmission electron microscopy images confirmed this rupture for radiant exposures of 25 mJ/cm² and above. Successful intracellular release was shown using the fluorescent dye calcein. For a radiant exposure of 35 mJ/cm² we found calcein delivery in 81 % of the treated cells while maintaining a high percentage of cell viability. Furthermore, cell proliferation and metabolic activity were not reduced 72 h after the treatment. Conclusion: CPPs trigger the uptake of the gold nanoparticle agglomerates via endocytosis and co-resident molecules in the endosomes are released by applying laser irradiation, preventing their intraendosomal degradation. Due to the highly localized effect, the cell membrane integrity is not affected. Therefore, this technique can be an efficient tool for spatially and temporally confined intracellular release. The utilization of specifically designed photodispersible gold nanoparticle agglomerates (65 nm) can open novel avenues in imaging and molecule delivery. Due to the induced deagglomeration the primary, small particles (~5 nm) are more likely to be removed from the body.
Organisationseinheit(en): Institut für Quantenoptik
Externe Organisation(en): Friedrich-Schiller-Universität Jena
Universität Duisburg-Essen
REBIRTH Forschungszentrum für translationale regenerative Medizin
Typ: Artikel
Journal: Journal of Nanobiotechnology
Band: 14
ISSN: 1477-3155
Publikationsdatum: 08.01.2016
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
ASJC Scopus Sachgebiete: Bioengineering, Medizin (sonstige), Molekularmedizin, Biomedizintechnik, Angewandte Mikrobiologie und Biotechnologie, Pharmazeutische Wissenschaften
Elektronische Version(en): https://doi.org/10.1186/s12951-015-0155-8 (Zugang: Offen)

BibTeX

@article{3aec3543648c478988565f1a777b1bac,
title = "Optical and electron microscopy study of laser-based intracellular molecule delivery using peptide-conjugated photodispersible gold nanoparticle agglomerates",
abstract = "Background: Cell-penetrating peptides (CPPs) can act as carriers for therapeutic molecules such as drugs and genetic constructs for medical applications. The triggered release of the molecule into the cytoplasm can be crucial to its effective delivery. Hence, we implemented and characterized laser interaction with defined gold nanoparticle agglomerates conjugated to CPPs which enables efficient endosomal rupture and intracellular release of molecules transported. Results: Gold nanoparticles generated by pulsed laser ablation in liquid were conjugated with CPPs forming agglomerates and the intracellular release of molecules was triggered via pulsed laser irradiation (λ = 532 nm, τpulse = 1 ns). The CPPs enhance the uptake of the agglomerates along with the cargo which can be co-incubated with the agglomerates. The interaction of incident laser light with gold nanoparticle agglomerates leads to heat deposition and field enhancement in the vicinity of the particles. This highly precise effect deagglomerates the nanoparticles and disrupts the enclosing endosomal membrane. Transmission electron microscopy images confirmed this rupture for radiant exposures of 25 mJ/cm2 and above. Successful intracellular release was shown using the fluorescent dye calcein. For a radiant exposure of 35 mJ/cm2 we found calcein delivery in 81 % of the treated cells while maintaining a high percentage of cell viability. Furthermore, cell proliferation and metabolic activity were not reduced 72 h after the treatment. Conclusion: CPPs trigger the uptake of the gold nanoparticle agglomerates via endocytosis and co-resident molecules in the endosomes are released by applying laser irradiation, preventing their intraendosomal degradation. Due to the highly localized effect, the cell membrane integrity is not affected. Therefore, this technique can be an efficient tool for spatially and temporally confined intracellular release. The utilization of specifically designed photodispersible gold nanoparticle agglomerates (65 nm) can open novel avenues in imaging and molecule delivery. Due to the induced deagglomeration the primary, small particles (~5 nm) are more likely to be removed from the body.",
keywords = "Cell-penetrating peptides, Endosomes, Gold nanoparticles, Intracellular molecule delivery, Laser-based release, Particle agglomerates",
author = "Judith Krawinkel and Undine Richter and Torres-Mapa, {Maria Leilani} and Martin Westermann and Lisa Gamrad and Christoph Rehbock and Stephan Barcikowski and Alexander Heisterkamp",
note = "Funding Information: The authors thank Dr. Hugo Murua Escobar, Hematology, Oncology and Pal‑ liative Medicine, University of Rostock, for providing the cells and biological counseling. We thank Prof. Dr. Anaclet Ngezahayo, Institute of Biophysics, Leibniz University of Hannover, for discussions about biological questions and Marinus Huber for performing the heat calculations. Thanks is also due to Christine K{\"a}mnitz for helping with and embedding the samples for TEM. Fur‑ thermore, we thank Ms. Maja Weber and Prof. Dr. Udo Markert, Placenta‑Lab of the Jena University Hospital for letting us use and helping with the handling of their SPECTROstar Omega plate reader. For financial support we thank the ”Deutsche Forschungsgemeinschaft (DFG)”, project Ba3580/10.",
year = "2016",
month = jan,
day = "8",
doi = "10.1186/s12951-015-0155-8",
language = "English",
volume = "14",
journal = "Journal of Nanobiotechnology",
issn = "1477-3155",
publisher = "BioMed Central Ltd.",
number = "1",
}