Chapter 3 Fungal Type I Polyketide Synthases

verfasst von: Russell J. Cox, Thomas J. Simpson
Abstract: Fungi produce a wide variety of biologically active compounds, a large proportion of which are produced by the polyketide biosynthetic pathway. Fungal polyketides comprise a very large and structurally very diverse group, and many display important biological activities, including lovastatin, aflatoxins, and strobilurins. These are produced by very large multifunctional iterative enzymes, the iterative type I polyketide synthases (PKSs) whose closest structural and functional analogues are the mammalian fatty acid synthases. Although fungal polyketides were one of the first classes of secondary metabolites to be subject to extensive biosynthetic studies, they remain the least studied and understood at the enzyme level. This chapter presents an overview of methodologies that have been applied to in vivo and in vitro genetic and biochemical studies on the PKSs responsible for both aromatic and highly reduced polyketide metabolites, and which are providing an improved insight into how these highly complex enzymes function.
Externe Organisation(en): University of Bristol
Typ: Beitrag in Buch/Sammelwerk
Seiten: 49-78
Anzahl der Seiten: 30
Publikationsdatum: 14.04.2009
Publikationsstatus: Veröffentlicht
Peer-reviewed: Ja
ASJC Scopus Sachgebiete: Biochemie, Molekularbiologie
Elektronische Version(en): https://doi.org/10.1016/S0076-6879(09)04603-5 (Zugang: Unbekannt)

BibTeX

@inbook{32925b90fecd4f6dab233fe1aa901fa3,
title = "Chapter 3 Fungal Type I Polyketide Synthases",
abstract = "Fungi produce a wide variety of biologically active compounds, a large proportion of which are produced by the polyketide biosynthetic pathway. Fungal polyketides comprise a very large and structurally very diverse group, and many display important biological activities, including lovastatin, aflatoxins, and strobilurins. These are produced by very large multifunctional iterative enzymes, the iterative type I polyketide synthases (PKSs) whose closest structural and functional analogues are the mammalian fatty acid synthases. Although fungal polyketides were one of the first classes of secondary metabolites to be subject to extensive biosynthetic studies, they remain the least studied and understood at the enzyme level. This chapter presents an overview of methodologies that have been applied to in vivo and in vitro genetic and biochemical studies on the PKSs responsible for both aromatic and highly reduced polyketide metabolites, and which are providing an improved insight into how these highly complex enzymes function.",
author = "Cox, {Russell J.} and Simpson, {Thomas J.}",
year = "2009",
month = apr,
day = "14",
doi = "10.1016/S0076-6879(09)04603-5",
language = "English",
isbn = "9780123745910",
series = "Methods in Enzymology",
number = "B",
pages = "49--78",
editor = "David Hopwood",
booktitle = "Complex Enzymes in Microbial Natural Product Biosynthesis, Part B",
edition = "B",
}