Optimized procedures for purification and solubilization of basic fibroblast growth factor inclusion bodies

verfasst von

D. Estapé, U. Rinas

Abstract

The efficiency of purification of basic fibroblast growth factor (bFGF) inclusion bodies using EDTA and nonionic detergents was improved from 25 to 40% by shifting the pH from 8.5 to strong alkaline conditions (pH 9.5 - 10.5). Complete dissolution of bFGF inclusion bodies by guanidinium hydrochloride (> 3 M) was independent of pH and the presence of reducing agents. In contrast, solubilization of bFCF inclusion bodies by urea was pH-dependent and increased in efficiency (e.g. from 0 to 100%) by increasing the pH (from pH 5.0 to 10.5 at 9 M urea). The purification and solubilization procedures are efficient for inclusion body concentrations corresponding to 10 and 100 g per I dry cell weight, respectively.

Externe Organisation(en)

Helmholtz-Zentrum für Infektionsforschung GmbH (HZI)

Typ

Artikel

Journal

Biotechnology Techniques

Band

10

Seiten

481-484

Anzahl der Seiten

4

ISSN

0951-208X

Publikationsdatum

1996

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Biochemie, Angewandte Mikrobiologie und Biotechnologie

Elektronische Version(en)

https://doi.org/10.1007/BF00159510 (Zugang: Geschlossen)